Scientists from the RIKEN Brain Science Institute have showed that a mice with a mutation that leads to dysfunction of mitochondria spontaneously undergo periodic episodes of depression-like behavior that resemble those in humans. Through this research, published in Molecular Psychiatry, the scientists have uncovered a link between depression and the paraventricular thalamus, a region of the brain not previously tied to depression.
“We decided to investigate where this was happening, and started experiments with a mouse strain with a mutation in Polg1, which is involved in the replication of mitochondrial DNA,” said Kato. Examining the behavior of these mutant mice, the researchers have discovered that the mice underwent spontaneous episodes where they would express signs and symptoms similar to the symptoms described in the DSM-5 criteria of major depressive episode in humans, similar to human patients with mitochondrial diseases,. "What we were surprised to find," says Dr. Takaoki Kasahara, one of the first authors of the study, “was that many of the female mutant mice showed symptoms similar to human depression patients. During the episodes, which began without reason and recurrently, the mice would run on their running wheels less extensively, showing they had a lack of pleasure-seeking behavior, a core feature of depression.”
The episodes tended to take place approximately every six months, beginning at the age of 30 weeks, and lasted for two or three weeks. When the mice were given an selective serotonin reuptake inhibitor—a common type of antidepressant drug—the episodes were mitigated, while lithium withdrawal induced episodes. The mice also had higher levels of corticosterone—an equivalent of cortisol in humans, which is often elevated in depressive patients. During the episodes, the mice showed signs such as weight gain, slow running speed, and increased fatigue, all of which are symptoms of depression in humans. The researchers then investigated which part of the brain was being affected by the mitochondrial abnormalities.
They found that there was a particularly high ratio of deleted mitochondrial DNAs in the paraventricular thalamus, a part of the brain that has not been previously linked to depression. When they examined brain slices from two deceased patients who had suffered from a mitochondrial disease coupled with mood symptoms, they found similar abnormalities in the paraventricular thalamus. To test the association, they blocked the transmission of signals from the paraventricular thalamus to other parts of the brain in mice, and found that they exhibited similar episodes.
“We are excited about this finding,” says Kato, “because this could lead to new therapies for depression, which takes a major health toll on society. Furthermore, this may represent a new mechanism for the onset of depressive episodes. It is also important because if we can show that depression can be caused by mitochondrial abnormalities in an area of the brain, it would allow us to reclassify it as a brain disease with a structural cause rather than a psychiatric illness.”